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cell ranger 6.0.1  (10X Genomics)

 
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    10X Genomics cell ranger 6.0.1
    Cell Ranger 6.0.1, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+ranger+6%2E0%2E1/pmc11874323__pnas__2424180122__sapp-194-11-14?v=10X+Genomics
    Average 90 stars, based on 1 article reviews
    cell ranger 6.0.1 - by Bioz Stars, 2026-07
    90/100 stars

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    Characterization of peripheral blood (PB) T cells in patients with RA and healthy donors (HDs) by ADTs and scRNA sequencing. (A) Experimental workflow including T cell enrichment and <t>10x</t> 5′ single cell coupled to ADT analysis and TCR and scRNA sequencing of three patients with RA and two HDs (created with Biorender.com). (B) UMAP displaying all TCRα/β + T cells in the analysis, color coded as indicated in the legend. Assignment of CD4 + and CD8 + T cells based on genomic RNA expression profiles. (C) Antibody‐derived tags (ADTs) for CD4 and CD8 represent CD4 and CD8 surface expression on T cells in representative dot plots of one HD and one patient with RA. (D) UMAPs showing distribution of CD4 and CD8 gene expression (upper row) and of ADT signal for CD4 and CD8 on T cells (lower row) of one representative donor. (E) Integrated data of ADT signals of five donors. Assignment of CD4 and CD8 positivity is according to labels derived from ADTs. Cells are color coded as indicated in the legend based on ADTs. Dots represent individual cells. RA, rheumatoid arthritis; scRNA, single‐cell RNA; TCR, T cell receptor; UMAP, uniform manifold approximation and projection.
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    Characterization of peripheral blood (PB) T cells in patients with RA and healthy donors (HDs) by ADTs and scRNA sequencing. (A) Experimental workflow including T cell enrichment and <t>10x</t> 5′ single cell coupled to ADT analysis and TCR and scRNA sequencing of three patients with RA and two HDs (created with Biorender.com). (B) UMAP displaying all TCRα/β + T cells in the analysis, color coded as indicated in the legend. Assignment of CD4 + and CD8 + T cells based on genomic RNA expression profiles. (C) Antibody‐derived tags (ADTs) for CD4 and CD8 represent CD4 and CD8 surface expression on T cells in representative dot plots of one HD and one patient with RA. (D) UMAPs showing distribution of CD4 and CD8 gene expression (upper row) and of ADT signal for CD4 and CD8 on T cells (lower row) of one representative donor. (E) Integrated data of ADT signals of five donors. Assignment of CD4 and CD8 positivity is according to labels derived from ADTs. Cells are color coded as indicated in the legend based on ADTs. Dots represent individual cells. RA, rheumatoid arthritis; scRNA, single‐cell RNA; TCR, T cell receptor; UMAP, uniform manifold approximation and projection.
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    Characterization of peripheral blood (PB) T cells in patients with RA and healthy donors (HDs) by ADTs and scRNA sequencing. (A) Experimental workflow including T cell enrichment and 10x 5′ single cell coupled to ADT analysis and TCR and scRNA sequencing of three patients with RA and two HDs (created with Biorender.com). (B) UMAP displaying all TCRα/β + T cells in the analysis, color coded as indicated in the legend. Assignment of CD4 + and CD8 + T cells based on genomic RNA expression profiles. (C) Antibody‐derived tags (ADTs) for CD4 and CD8 represent CD4 and CD8 surface expression on T cells in representative dot plots of one HD and one patient with RA. (D) UMAPs showing distribution of CD4 and CD8 gene expression (upper row) and of ADT signal for CD4 and CD8 on T cells (lower row) of one representative donor. (E) Integrated data of ADT signals of five donors. Assignment of CD4 and CD8 positivity is according to labels derived from ADTs. Cells are color coded as indicated in the legend based on ADTs. Dots represent individual cells. RA, rheumatoid arthritis; scRNA, single‐cell RNA; TCR, T cell receptor; UMAP, uniform manifold approximation and projection.

    Journal: Arthritis & Rheumatology (Hoboken, N.j.)

    Article Title: CD4 + CD8α low T Cell Clonal Expansion Dependent on Costimulation in Patients With Rheumatoid Arthritis

    doi: 10.1002/art.42960

    Figure Lengend Snippet: Characterization of peripheral blood (PB) T cells in patients with RA and healthy donors (HDs) by ADTs and scRNA sequencing. (A) Experimental workflow including T cell enrichment and 10x 5′ single cell coupled to ADT analysis and TCR and scRNA sequencing of three patients with RA and two HDs (created with Biorender.com). (B) UMAP displaying all TCRα/β + T cells in the analysis, color coded as indicated in the legend. Assignment of CD4 + and CD8 + T cells based on genomic RNA expression profiles. (C) Antibody‐derived tags (ADTs) for CD4 and CD8 represent CD4 and CD8 surface expression on T cells in representative dot plots of one HD and one patient with RA. (D) UMAPs showing distribution of CD4 and CD8 gene expression (upper row) and of ADT signal for CD4 and CD8 on T cells (lower row) of one representative donor. (E) Integrated data of ADT signals of five donors. Assignment of CD4 and CD8 positivity is according to labels derived from ADTs. Cells are color coded as indicated in the legend based on ADTs. Dots represent individual cells. RA, rheumatoid arthritis; scRNA, single‐cell RNA; TCR, T cell receptor; UMAP, uniform manifold approximation and projection.

    Article Snippet: Count matrixes have been created with 10x Genomics Cell Ranger (version 6.0.1) with the count function from raw data.

    Techniques: Sequencing, RNA Expression, Derivative Assay, Expressing